In some cases, we might not be able to provide certain products (depends on ourĪgreement with the main supplier or supplier policy that LaboShop must follow), this willīy clicking ( send inquiry), it doesn’t mean any obligationįrom our side to supply the selected item, or an obligation from your side to buy (no Our services cover the Middle East and Arab countries ONLY. LaboShop might apply extra charges for installation and delivery unless it is shown.The product to LaboShop in this case, all related expenses will be deducted from the customer (storage, damage, shipping/returning, etc.). It is the customer’s responsibility to provide any kind of documents and/or localĪpprovals that might be required by customs, such as MOH approval or any document related to customer’s lab.Īny failure in providing the required documents within 6 working days means returning (official receipt will be given to you to be cleared before delivery). Value of taxes, VAT, and customs duties are the customer’s responsibility if applicable Installation and delivery of the products to your door are LaboShop’s responsibility to any location in MENAĪll prices shown online do not include any local taxes, VAT, or customs duties. Wide selection of restriction endonuclease specificities.Includes universal Tango buffer for double-digestions.Convenient color-coded Five Buffer System.Superior quality-stringent quality control and industry leading manufacturing process.To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. In addition, the universal Tango buffer is provided for convenience in double digestions. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. Note: Also available as a FastDigest enzyme for rapid DNA digestion. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Thermo Scientific NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37☌ in O buffer (Isoschizomers: CciNI). To avoid these, follow the recommended guidelines for storage and reactions, and always check for the efficacy of digestion along with purification of digested products on an agarose gel.Thermo Scientific™ NotI (10 U/µL), 1,500 units Star activity (or off-target cleavage) and incomplete cleavage are potential challenges which may occur due to suboptimal enzymatic conditions or inappropriate enzyme storage. For complete digestion, make sure that the enzyme volume is 1/10th of the total reaction volume, the optimal temperature is constantly maintained throughout the reaction, the total reaction time is appropriately calculated based on the amount of DNA to be digested, appropriate buffers should be used to ensure maximal enzymatic activity, and in case of a double digest, make sure that the two restriction sites are far enough so that the activity of one enzyme cannot interfere with the activity of the other. Always use a control DNA digestion with the enzyme to ensure adequate activity (to avoid interference due to high glycerol in the enzyme). The enzyme should always be stored at -20C and multiple freeze-thaw cycles should be avoided in order to maintain optimal activity. The most common challenges with restriction digest include- 1. The four most common types of restriction enzymes include: Type I (cleaves at sites remote from a recognition site), Type II (cleaves within or at short specific distances from a recognition site), Type III (cleave at sites a short distance from a recognition site), and Type IV (targets modified DNA- methylated, hydroxymethylated and glucosyl-hydroxymethylated DNA). Restriction Enzymes NotI A restriction enzyme or restriction endonuclease is defined as a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at or near that site, known as restriction site or target sequence.
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